Evaluation of Genetic Variability between Local White Chicken and Commercial Lines by RAPD-PCR and Sequencing of 18s rRNA Gene

The present study investigated and identified genetic diversity between native White chicken lines two commercial Broiler (Rose) Layer (Isa Brown) breeds using RAPD markers a sequencing technique. All primers applied produced 151 scorable bands with percentage polymorphic loci of 54.93 % within populations, as per the results marker. maximum amplified fragment by primer OPC-11 was 22 fewest OPAA-03 7. For all analyzed, effective number alleles (ne), means observed (na), Shannon's information index (I), gene (h) 1.4394, 1.5493, 0.3496, 0.2441, respectively. presence high polymorphisms targeted (71) across populations indicates that RAPD-PCR techniques provide sufficient distance higher variation among populations. highest identity blasted sequences 18srRNA local white is 90.41 84.23 %. Likewise, total 46 27 nucleotides are altered 10 gaps in both for first second regions, According to phylogenetic trees, had stronger sense individuality slightly closer broiler than layer breeds. As result, it suggests enhancing line requires breeding program, well cross-breeding other obtain hereditarily significant new strains.